Molecular Genetics
Compulsory subject
- in BSc Applied Biology, 3rd Semester
- 6h/week (4L/2P)
- Credits: 7 ECTS
Lecture
Learning outcomes:
At the end of the lecture the students are able:
- to know the most important molecular genetic processes in the cell, such as replication, regulation, variation, transcription, translation and expression, cell cycle and repair mechanisms
- to know the most important molecular genetic events in gene technology
- to judge ethical aspects of molecular genetics
Content:
- Molecular structure and function of DNA and RNA
- Replication, transcription and translation: Mechanisms and enzymes involved
- Comparison of replication and gene expression in prokaryotes, eukaryotes and viruses: Similarities and differences
- Regulation of gene expression in prokaryotes, viruses and eukaryotes
- Variation and mutation
- Repair mechanisms
- The eukaryotic chromosome
- The eukaryotic cell cycle
- Mitosis and meiosis
- Transposable elements
- Profiling and polymorphisms
- Gene technology as applied molecular genetic: Techniques,enzymes, application
- Ethical aspects of molecular genetics
- Composition, structure and reproduction of the most important virus families
Practical Course
Learning outcomes:
At the end of the laboratory course the students are able:
- to isolate, characterise and analyse genomic and plasmid DNA
- to perform bacterial gene transfer and bacteriophage titration
Content:
- Bacterial Conjugation
- Bacteriophage Titering
- Isolation of Plasmid DNA by HiSpeed Plasmid Mini-Preparation
- Determination and Characterisation of Plasmid DNA
- Isolation of Human Genomic DNA from Buccal Swabs
- Typing of Human Genomic DNA
Prerequisites
- Enrolment in the 3. semester of the Applied Biology program at Bonn-Rhein-Sieg University of Applied Sciences.
-
Successful attendance of the General Safety Instructions (part of the Introduction Weeks [3], always in September/October) and the Special Safety Instructions (S1).
Information For New Students: You have to attend these instructions before you are allowed to participate in any practical course of this university. Please ask for the dates in advance!
Recommendations:
- Passing of the modules of the 1st and 2nd semester
Assessment
Successful participation in Written Exam and in Practical Course.
Written Exam: 70%
Practical Course: 30%
Literature
- Lewin Genes VIII, Pearson Verlag
- Griffiths, Gelbart, Miller, Lewontin; Modern Genetic Analysis; Freeman and Company
- D. M. Knipe and P. M. Howley; Fields Virology; Lippincott Williams & Wilkins
- Birge; Bacterial and Bacteriophage Genetics; Springer Verlag Alberts, Bray, Lewis, Raff, Roberts, Watson, Molekularbiologie der Zelle, VHC Verlagsgesellschaft
- Clark, Molecular Biology, Understanding the Genetic Revolution Kippers, Molekulare Genetik, Thieme Verlag
- Nicholl; Gentechnische Methoden; Spektrum Verlag (German)
- Henning; Genetik, Springer Verlag (German)
- Lewin; Molekularbiologie der Gene; Spektrum Verlag (German)