Impact of MSCs Differentiating Towards Adipocytes on Endothelial Gene Expression
Juliane Czeczor (BSc Applied Biology)
Diabetes type 2 is the predominantly occurring form of diabetes with an increasing prevalence. A typical sequel is atherosclerosis subsequently followed by stenosis, stroke, and heart attack causing about half of the deaths of adults over age 60 in industrialized countries and being the main cause of death of diabetes type 2 patients.
Atherosclerosis evolves due to fatty degeneration and calcification of arteries. This leads to plaque formation that represents a potential risk for the blood circulation by occlusion, narrowing of arteries and eventually the rupture of the vessel.
A major factor contributing to atherosclerosis is the developing adipositas.
In this research project we will investigate the effect of the co-cultivation of human mesenchymal stem cells (hMSC) differentiating and differentiated to adipocytes, on endothelial cells of various organisms. To do so, parameters such as adhesion molecules, glucose transporter molecules (GLUT), second messengers, matrix metalloproteinases, and the activation of PPARγ will be elucidated on mRNA and protein level.
Understanding the interactions between adipocytes and endothelial cells will reveal new insights in the pathogenesis of atherosclerosis. In addition, obtained results might contribute to future treatment approaches and new diagnostic tools for the disease.