Genetic Engineering

Compulsory Course

  • in  BSc Applied Biology, 5th Semester
  • 6h/week (2L/2E/2P)
  • Credits: 6 ECTS

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Hochschule Bonn-Rhein-Sieg

Lecture

Learning outcomes:

After successful attendance of the lecture and exercise "Genetic Engineering" students will be able to:

  • understand and explain the basics of applications of genetic engineering.
  • Students are familiar with the general procedure, methods and techniques involved in molecular cloning and are able to explain their molecular or biochemical background.
  • Students know different types of vectors used in genetic engineering and can evaluate their use for different cloning purposes.
  • Students know about the significance of gene libraries and are familiar with the procedures involved in constructing them.
  • Techniques involved in the generation of transgenic plants or animals are known, current applications can be mentioned and explained.
  • Moreover students are able to interprete and evaluate current scientific research and applied technologies in the field of genetic engineering.

Content:

  • Basics of working with recombinant DNA; cloning techniques
  • Restriction endonucleases
  • Ligases
  • Heterologous gene expression
  • Vectors and plasmids
  • Transformation of bacterial cells
  • Cloning of a DNA molecule into a plasmid vector
  • Analysis of recombinant DNA
  • Screening methods
  • Hybridisation of nucleic acids
  • Applications of the genetic engineering technology in the biotechnology field

Exercise

Content:

  • Solving problems and questions developing from the lectures issues
  • Working on literature from the field

Practical Course

Learning outcomes:

After successful attendance of the practical course students are able to:

  • produce and molecularly clone in vitro recombined nucleic acids in a bacterial host
  • identify molecular clones and analyse the expression of a cloned gene

Content:

  • Molecular cloning of a PCR fragment in E.coli using an expression vector
  • Ligase reaction of the fragment with the vector molecule
  • Generation and use of competent cells
  • Generation of bacterial clones
  • Analysis for the presence of the cloned gene fragment and of its expression in RNA and enzymatic activity, respectively

Prerequisites

none

Recommendations: Successful attendance of the modules "Biochemistry" und "Molecular Genetics"

Assessment

The final mark for this module is determined by:

  • A written exam on the subjects of the lecture and the exercises (50% of the final mark), in which at least 50 % of the potential maximum score has to be reached.
  • A lab report on the practical work (50% of the final mark)

Literature

  • Sambrook, Fritsch, Maniatis (2003): Molecular Cloning, a Laboratory Manual Vol.1, 2, and 3.; Cold Spring Harbor Laboratory Press.
  • Primrose and Twyman (2006): Principles of Gene Manipulation and Genomics; Blackwell Pubishing
  • Nicholl (2008): Genetic Engineering; Cambridge University Press
  • Howe (2007): Gene Cloning and Manipulation; Cambridge University Press