Department of Natural Sciences
Cell culture
Lecture
Compulsory course
- in BSc Applied Biology, 4th Semester
- 3 h/week (1L/2P)
- Credits: 3 ECTS
Learning outcomes:
At the end of the lecture the students are able:
- to know the cell culture equipment
- to know how to get information about cell lines and order them
- to discriminate cell lines from primary cells and lab scale from technical scale
- to recognize chromosome banding patterns
- to discriminate contaminations and their sources
- to know the basic of apoptosis
Content:
- Basics of cell- and tissue culture; appropriate handling of cell culture equipment
- Sterile techniques and sterile working
- Biology of cultivated cells; cultivation of primary cells; cloning and characterization of cell lines; cultivation and sub cultivation of cell lines; techniques of quantification of eukaryotic cells
- “Scale-Up” of cell cultures
- Contaminations in cell culture: Detection, control and prophylaxis
- Transformation of cells and tumour cells
- Stem cells and differentiation
- Karyotyping
- Cell death: apoptosis and necrosis
Internship
Learning outcomes:
At the end of the laboratory course the students are able:
- to use the cell culture equipment correctly
- to work sterile in cell culture
- to in vitro cultivate and sub cultivate monolayer and suspension cells
- to recognize and test for contaminations
- to perform in vitro gene transfer into eukaryotic cells
Content:
- Permanent cell culture of monolayer and suspension cells with splitting, freezing and thawing of the cells
- RT-PCR and nested PCR for detection of mycoplasma infection
- Gene transfer into eukaryotic cells and use of reporter genes
Requirements
General Safety Instruction
Recommendations: Passing of the modules of the 1st, 2nd and 3rd semester
Passing of module – graded
The final mark of the module consists of:
- a written test composed of questions to the content of the lecture and the provided protocol for the practical part. The written test must be passed with at least 50 % of the possible points and counts 50 % of the final mark.
- the correct execution of the experiments in the laboratory which counts 20 % of the final mark.
Writing the minutes of the experiments which counts 30 % of the final mark.
Literature
- R.I. Freshney; Culture of Animal Cells; ISBN 0-471-34889-9; Wiley-Liss
- J. Walker; Methods in Molecular Biology: Basic Cell Culture Protocols; ISBN 0-89603-384-8; Humana Press
- T. Lindl; Zell- und Gewebekultur (6th ed.); ISBN 3-82-74-1194-7; Spektrum Verlag
Links
Further links