Compulsory Course

  • in BSc Applied Biology, 4th Semester 
  • 6h/week (2L/2E/2P)
  • Credits: 7 ECTS

→Download scripts, group assignment etc. (LEA)

Hochschule Bonn-Rhein-Sieg


Learning outcomes:

At the end of the study unit lecture/exercise the students are able:

  • to explain the origin of immunological cells from the bone marrow and thymus
  • to understand differentiation and activation of immunological cells
  • to understand the reaction of the immune system to infections


  • Basics of the immune system; knowledge of immunological techniques: Components of the immune system, immunological organs, structure and function of organs, cells of the immune system
  • Innate immune systeme:Protective function of skin and mucosal tissues, phagocytes, complement system, natural killer cells, cytokines, chemokines, inflammatory reaction
  • Adaptive immunsystem: B-cell system, ´receptors, antigen recognition, antibody production, functions of antibodies;
  • T-cell-system: receptors, antigen recognition, effetor cells, mechanisms of cellular immunity; immunological memory. signal transduktion in the immune system


The exercise serves to discuss the content of the lecture based on questions.

Both have the same content.

Practical Course

Learning outcomes:

At the end of the practical course the students are able:

  • to work with blood cells and analyze these in blood smear
  • to apply FACS technology towards the allergen specific degranulation of basophils
  • to sort lymphocyte subsets via magnetic cell sorting
  • to isolate immune cells from different organs and analyze them
  • to test blood groups


  • Magnetic sorting of cells
  • Degranulation of Basophils with allergens
  • Determination of blood groups, blood picture
  • Flow cytometry
  • Isolation of primary lymphocytes
  • Determination of blood groups



Recommendations: Biology 1-4; Biochemistry


Passing of module – graded

The total grade of the module is comprised of:

  1. a written exam about the content of the lecture and the practical course, which has to be passed with at least 50% of the total points. The grade of the exam contributes by 70% to the total grade of the module
  2. writing an individual laboratory report. Grading of the lab report contributes by 30% to the total grade of the module


  • Immunobiology. C.A. Janeway, P. Travers, M. Walport and J.D. Capra, latest edition