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Department of Natural Sciences

Instrumental Analysis

Lecture

Compulsory Course

  • in BSc Applied Biology, 3rd Semester
  • 6h/week (2L/3E/1P)
  • Credits: 7 ECTS

 

 

Learning outcomes:

Students have acquired knowledge about the basic principles of common electrophoretic and chromatographic separation techniques as well as about the fundamentals of UV-Vis spectroscopy and mass spectrometry. They know major application areas of those techniques, their advantages or shortcomings for certain separation problems and the required technical appliances.

Content:

  • Fundamentals and basic applications of gel and capillary electrophoresis, UV-Vis spectroscopy and mass spectrometry
  • Fundamentals and major applications of HPLC, GC, TLC, ion exchange and size exclusion chromatography, basic principles of method development by means of simple examples
  • Presentation of relevant sample preparation techniques (such as dialysis, ultrafiltration, solid-phase extraction and liquid-liquid extraction)

Exercises

Learning outcomes:

Students are able to assess the significance of different experimental parameters and their effects on the results of a separation technique. They know how to develop suitable methods for specified simple separation problems, how to carry out stoichiometric calculations for sample preparation and how to determine experimental parameters by evaluating simple chromatograms or spectra. 

Internship

Learning outcomes:

They gained practical experience with gel electrophoresis, UV-Vis spectroscopy and/or chromatography. They know how to operate the technical appliances after instructions by a tutor, and they are capable of evaluating gels, spectra or chromatograms 

Content:

Laboratory experiments regarding gel electrophoresis (SDS-PAGE), UV-Vis spectroscopy and/or chromatography (e.g. HPLC)

Requirements

none

Recommendations: Mathematics (1st Sem.), General Chemistry (1st Sem.), Physics/Statistics (2nd Sem.)

 

Passing of module – graded

Practical course (written tests and reports): 20%

Written examination: 80%.

Practical course and written examination have to be passed independently.

Literature

  • F. Lottspeich, J.W. Engels, Bioanalytik, Spektrum Akademischer Verlag.
  • D.A. Skoog, F.J. Holler, T.A. Nieman, Principles of Instrumental Analysis, Brooks / Cole Thomson Learning.
  • A. Manz, N. Pamme, D. Iossifidis, Bioanalytical Chemistry, Imperial College Press.
  • R. Westermeier, Electrophoresis in Practice, Wiley.
  • M. Hesse, H. Meier, B. Zeeh, Spectroscopical Methods in Organic Chemistry, Thieme Verlag.
  • L.R. Snyder, J.J. Kirkland, J.L. Glajch, Practical HPLC method development, Wiley.
  • M. Kinter, N.E. Sherman, Protein Sequencing and Identification Using Tandem Mass Spectrometry, Wiley.
  • R.L. Grob, E.F. Barry, Modern Practice of Gas Chromatography, Wiley.
  • E. Hahn-Deinstrop, Applied Thin Layer Chromatography, Wiley-VCH.

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