Department of Natural Sciences

Forensic Biology

Lecture

Compulsory course in the 3rd semester of Forensic Sciences

The course consists of:
Lecture: 4 lesson hours per week
Laboratory course: 2 lesson hours per week;

7 ECTS

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Application of forensic DNA profiling; identifying biological evidence;
DNA isolation methods; short history of forensic biology; STR systems
and their analysis via multiplex PCR and capillary electrophoresis;
population genetics, databases and calculation of random match
probabilities; qPCR; profiling of mtDNA; paternity testing; Y-STRs;
forensic species determination (Cytb, COI, STRs)

Download scripts, exercises, group assignment etc (LEA)

Practical Course

DNA isolation from blood and buccal swabs; DNA quantitation using realtime
PCR; STR analysis using multiplexPCR and capillary electrophoresis; mtDNA-based
determination of meat species; forensic sex typing (amelogenin)

Download scripts, exercises, group assignment etc (LEA)

Requirements

Enrolment in the 3. semester of the programme "Bachelor of Science in Naturwissenschaftlicher Forensik" at the University of Applied Sciences Bonn-Rhein-Sieg.
Successful attendance of the General Safety Instructions (part of the Introduction Weeks, always in September/October) and the Special Safety Instructions given in the 2. semester (see Fundamental Biology-Course).

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Modular examination – graded
Laboratory work (oral examinations and lab reports): 33%;
Written final examination: 67%
Active participation in the laboratory course is a prerequisite for
admission to the final examination.
The final examination must be passed independently of the practical
part.

Literature

John M. Butler: Fundamentals of Forensic DNA Typing (Elsevier)
William Goodwin, Adrian Linacre, Sibte Hadi: An Introduction to Forensic Genetics, 2nd Edition (Wiley-Blackwell)