Research:

Applied, environmental and clinical microbiology, microbial genetics and genomics

Methods:

PCR (qRT-PCR, 16SrRNA-PCR, inverse PCR, long-range PCR), multilocus sequence typing, STR typing, next generation sequencing (Illumina Miseq), whole genome mapping (Optical Mapping, Argus, Opgen), DNA fragment analysis (Tape Station, Agilent), genome analysis, bioinformatics (CLC Genomics and Geneious), proteomics, flow cytometry, fluorescence microscopy, fluorescence in situ hybridization

Teaching:

NGS elective courses (Parts 1 & 2) in MSc Biomedical sciences, 2nd and 3rd Semesters

Content: 

• Fundamentals of NGS
• Assessing quality of sequence runs and sequence data
• 16-rRNA metagenomic sequencing
• Shotgun metagenomic sequencing
• Whole genome sequencing
• Targeted amplicon and panel sequencing
• Forensics NGS
• RNA-seq