Experimental Models

The World in the Lab

Mammalian Cell Culture

Areas of expertise

- Metabolism
- Enzymology
- Protein localization
- In vitro models for epithelial barriers
- Immune cell differentiation in health and disease

Contact: Prof. Dr. Mike Althaus; Prof. Dr. Jörn Oliver Sass, Prof. Dr. Harald Illges

Experimental Models Mammalian Cell Culture

Current research topics

- Inborn errors of metabolism
- Metabolism of branched-chain amino acids and ketone bodies
- Biochemistry of acylation and deacylation
- Pathobiochemistry
- Rare diseases
- Autoimmunity
- CD21

Caenorhabditis elegans

Areas of expertise

- Metabolism
- Enzymology
- Caenorhabditis elegans life span and behavior
- Localization studies

Contact: Prof. Dr. Jörn Oliver Sass

Experimental Models Caenorhabditis Elegans

Current research topics

- Caenorhabditis elegans as an invertebrate animal model for metabolic diseases
- Toxicity studies

Nematode C. elegans

H-BRS

Xenopus laevis oocytes

Areas of expertise

- Cell signaling
- Membrane transport
- Heterologous expression of ion channels and transporters

Contact: Prof. Dr. Mike Althaus

Experimental Models Xenopus Laevis Oocytes

Current research topics

- Molecular physiology of ion channels and transporters
- Channelopathies and personalized medicine

Technical Infrastructure

- Seahorse® for study of energy metabolism
- UV/Vis, Fluorescence, Luminescence and detection for enzyme characterization
- Fluorescence Polarization Analysis
- Confocal Laser Scanning Microscopy
- Fluorescence Microscopy
- FACS/MACS
- Patch-Clamp electrophysiology
- Two-electrode Voltage-Clamp electrophysiology (automated, Roboocyte2)
- Ussing-chamber electrophysiology
- Live Imaging